ELISA Antibody Detection Tests: Mannheimia haemolytica Antibody ELISA (Cattle)

Mannheimia haemolytica is a significant pathogen in cattle, primarily associated with respiratory disease, particularly bovine respiratory disease (BRD). This bacterium can cause severe clinical symptoms, leading to substantial economic losses in the cattle industry due to decreased productivity and increased veterinary costs. Using an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Mannheimia haemolytica offers a reliable method for diagnosing infections and managing herd health.

Importance of Antibody Detection

Detecting antibodies against Mannheimia haemolytica helps identify animals exposed to the pathogen, even if they are not showing clinical signs. This is particularly important in managing BRD, as early identification can lead to more effective treatment and control measures. Understanding the immune response within a herd can guide vaccination strategies and enhance overall herd immunity. Animals that have recently developed antibodies are likely to have recently been affected by bovine pneumonia.

ELISA Methodology

Serum samples from cattle are tested in the laboratory on antigen-coated ELISA plates. The process involves several incubation steps, after which a colour change gives an indication of the level of antibodies present in the sample.

Applications

Mannheimia haemolytica antibody ELISA tests have multiple applications in cattle health management:

  • Disease Surveillance –  Regular testing can help monitor the prevalence of Mannheimia haemolytica in herds, enabling proactive management strategies.
  • Vaccination Efficacy –  ELISA can assess the immune response to vaccines, determining whether animals have developed sufficient antibodies post-vaccination.
  • Outbreak Investigation –  During BRD outbreaks, ELISA can identify affected animals, providing prompt intervention and treatment.
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