Salmonella Antibody ELISA (Multispecies)
Salmonella is a significant zoonotic pathogen that risks both animal and human health. In cattle, Salmonella infections can lead to enteritis, systemic disease, and significant economic losses due to decreased productivity and increased veterinary care costs. The Salmonella antibody ELISA test is a valuable diagnostic tool for detecting antibodies in various animal species, helping to monitor and control Salmonella infections in livestock populations.
Importance of Antibody Detection
Detecting antibodies against Salmonella provides insights into the immune response of animals following exposure to the pathogen, aiding in understanding the epidemiology of Salmonella infections. It also helps identify carriers that may not exhibit clinical signs but can shed the bacteria, contributing to the spread of the disease within and beyond the herd. The test can also be used to assess the response to vaccination
ELISA Methodology
Blood samples are taken in plain (clotted) tubes and sent to the laboratory. The samples are spun down to allow serum harvesting. The level of antibody present in the sample is determined by measuring the optical density of the samples following a number of incubation steps. Samples can be specifically tested to detect antibodies to Salmonella Dublin or to a broad range of Salmonella spp.
Applications
The Salmonella antibody ELISA has various applications in livestock health management:
- Disease Surveillance: The test is used for routine monitoring of Salmonella infections in herds, enabling early intervention.
- Food Safety: By identifying carriers, the ELISA test helps reduce the risk of Salmonella transmission through the food chain, ensuring safer meat and dairy products.
- Vaccination Efficacy Assessment: The test can evaluate the immune response to vaccines designed to protect against Salmonella infections.
